Detailed information of TA system TA214151

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1941463..1942298
• Replicon: chromosome
• Accession: NZ_CP082127
• Organism: Escherichia coli strain NDM_12_14482

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1U9SHU4
• Locus tag: K5P76_RS09260
• Protein ID: WP_000854752.1
• Coordinates: 1941463..1941840 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: K5P76_RS09265
• Protein ID: WP_001278232.1
• Coordinates: 1941930..1942298 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P76_RS09225 (1936983)	1936983..1937456	+	474	WP_001105384.1	DNA gyrase inhibitor SbmC	-
K5P76_RS09230 (1937654)	1937654..1938712	+	1059	WP_001200892.1	FUSC family protein	-
K5P76_RS09235 (1938884)	1938884..1939213	+	330	WP_000450409.1	DUF496 family protein	-
K5P76_RS24405 (1939314)	1939314..1939679	-	366	WP_223350516.1	EutP/PduV family microcompartment system protein	-
K5P76_RS09245 (1939950)	1939950..1940321	-	372	WP_001295631.1	IS110 family transposase	-
K5P76_RS09250 (1941137)	1941137..1941217	-	81	Protein_1825	hypothetical protein	-
K5P76_RS09255 (1941317)	1941317..1941466	-	150	Protein_1826	DUF5983 family protein	-
K5P76_RS09260 (1941463)	1941463..1941840	-	378	WP_000854752.1	TA system toxin CbtA family protein	Toxin
K5P76_RS09265 (1941930)	1941930..1942298	-	369	WP_001278232.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
K5P76_RS09270 (1942461)	1942461..1942682	-	222	WP_000692345.1	DUF987 domain-containing protein	-
K5P76_RS09275 (1942745)	1942745..1943221	-	477	WP_001186774.1	RadC family protein	-
K5P76_RS09280 (1943435)	1943435..1944976	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
K5P76_RS09285 (1944991)	1944991..1945737	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
K5P76_RS09290 (1945823)	1945823..1946296	-	474	WP_000855059.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14076.08 Da
• Isoelectric Point: 8.2905

>T214151 WP_000854752.1 NZ_CP082127:c1941840-1941463 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T214151 NZ_CP109984:41436-41723 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT214151 WP_001278232.1 NZ_CP082127:c1942298-1941930 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT214151 NZ_CP109984:41197-41436 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A1U9SHU4

Antitoxin

• AlphaFold DB: E3PJ72