Detailed information of TA system TA021415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 3165964..3166547
• Replicon: chromosome
• Accession: NC_007777
• Organism: Frankia sp. CcI3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q2J9K1
• Locus tag: FRANCCI3_RS13500
• Protein ID: WP_011437076.1
• Coordinates: 3166260..3166547 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q2J9K2
• Locus tag: FRANCCI3_RS13495
• Protein ID: WP_011437075.1
• Coordinates: 3165964..3166263 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRANCCI3_RS13485	3161134..3165210	-	4077	WP_108913731.1	DISARM system helicase DrmA	-
FRANCCI3_RS26260	3165614..3165709	-	96	Protein_2742	SAM-dependent methyltransferase	-
FRANCCI3_RS13495	3165964..3166263	+	300	WP_011437075.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FRANCCI3_RS13500	3166260..3166547	+	288	WP_011437076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FRANCCI3_RS13505	3166901..3167431	-	531	Protein_2745	transposase	-
FRANCCI3_RS13510	3167464..3168789	-	1326	Protein_2746	IS607 family element transposase accessory protein TnpB	-
FRANCCI3_RS13515	3168786..3169358	-	573	WP_011437080.1	IS607 family transposase	-
FRANCCI3_RS24635	3169416..3169914	-	499	Protein_2748	helix-turn-helix domain-containing protein	-
FRANCCI3_RS13520	3169928..3170332	-	405	Protein_2749	IS200/IS605 family transposase	-
FRANCCI3_RS27335	3170456..3170791	+	336	WP_193359370.1	hypothetical protein	-
FRANCCI3_RS23505	3171068..3171319	-	252	Protein_2751	SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (27-92)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10664.26 Da
• Isoelectric Point: 10.0089

>T21415 WP_011437076.1 NC_007777:3166260-3166547 [Frankia casuarinae]
MTGAAGPYRLEITGPAARALAGRIPEKVATAVHEFITTTLLENPHRLGKRLLYPPYAGTWSARRGMYRVLYEIDEENRIV
LVTAVEHRADAYRGR

Nucleotide

• Length: 288 bp

>T21415 NC_007777:3166260-3166547 [Frankia casuarinae]
ATGACGGGCGCTGCCGGGCCGTACCGTCTTGAGATCACCGGTCCGGCGGCCCGCGCGCTTGCTGGACGTATACCGGAAAA
GGTCGCCACGGCCGTACACGAGTTCATCACCACGACGCTCCTGGAGAACCCGCACAGGCTCGGTAAGCGTCTCCTCTACC
CGCCCTACGCTGGAACCTGGTCGGCGCGGCGAGGCATGTACCGGGTGCTCTATGAGATTGACGAAGAGAACCGCATCGTT
CTCGTGACCGCCGTAGAGCACCGCGCCGACGCATACCGCGGGCGTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10889.15 Da
• Isoelectric Point: 4.6155

>AT21415 WP_011437075.1 NC_007777:3165964-3166263 [Frankia casuarinae]
VTTLPFTDARNRLSELLDDVERTHDRVEITRHGHAVAILVSPDDLAALEETVEVLSNRETMRQLAESRAAVEAGDVLDAE
DLAALMAKRAAPDARRRSS

Nucleotide

• Length: 300 bp

>AT21415 NC_007777:3165964-3166263 [Frankia casuarinae]
GTGACGACGCTGCCCTTCACGGATGCTCGGAACCGCCTCTCGGAGCTGCTCGACGACGTCGAGCGAACCCATGACCGGGT
AGAGATCACACGCCATGGTCATGCCGTGGCGATCCTCGTCTCGCCTGACGACCTCGCGGCGCTGGAGGAGACCGTCGAGG
TGTTGTCGAACCGCGAGACCATGCGGCAGTTGGCCGAGTCCCGCGCCGCTGTCGAGGCCGGCGACGTGCTTGACGCCGAG
GACCTGGCGGCGCTCATGGCCAAGCGCGCCGCGCCGGATGCCAGGAGACGCTCGTCATGA

Structures

Toxin

• AlphaFold DB: Q2J9K1

Antitoxin

• AlphaFold DB: Q2J9K2