Detailed information of TA system TA021414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 3017911..3018425
• Replicon: chromosome
• Accession: NC_007777
• Organism: Frankia sp. CcI3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q2J9X3
• Locus tag: FRANCCI3_RS12860
• Protein ID: WP_011436954.1
• Coordinates: 3018165..3018425 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q2J9X4
• Locus tag: FRANCCI3_RS12855
• Protein ID: WP_011436953.1
• Coordinates: 3017911..3018168 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FRANCCI3_RS26240	3013238..3014242	+	1005	WP_023841607.1	hypothetical protein	-
FRANCCI3_RS12835	3014471..3015742	-	1272	WP_108913874.1	recombinase family protein	-
FRANCCI3_RS12840	3015739..3016071	-	333	Protein_2607	hypothetical protein	-
FRANCCI3_RS24590	3016230..3016868	+	639	Protein_2608	transposase family protein	-
FRANCCI3_RS12850	3016826..3017440	+	615	WP_082456841.1	reverse transcriptase	-
FRANCCI3_RS26980	3017580..3017717	-	138	WP_023842113.1	hypothetical protein	-
FRANCCI3_RS12855	3017911..3018168	+	258	WP_011436953.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FRANCCI3_RS12860	3018165..3018425	+	261	WP_011436954.1	Txe/YoeB family addiction module toxin	Toxin
FRANCCI3_RS26985	3018603..3018746	-	144	WP_023842114.1	hypothetical protein	-
FRANCCI3_RS12865	3019577..3020329	+	753	WP_198031069.1	DJ-1/PfpI family protein	-
FRANCCI3_RS27505	3020286..3020552	+	267	WP_198031070.1	helix-turn-helix domain-containing protein	-
FRANCCI3_RS12870	3020552..3021192	+	641	Protein_2616	DJ-1/PfpI family protein	-
FRANCCI3_RS12875	3021365..3022003	+	639	WP_011436957.1	DUF2867 domain-containing protein	-
FRANCCI3_RS12880	3022343..3023122	-	780	WP_011436958.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3010667..3031936	21269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-70)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10222.58 Da
• Isoelectric Point: 8.8808

>T21414 WP_011436954.1 NC_007777:3018165-3018425 [Frankia casuarinae]
VKLTWTDHAWDDYLYWQSQDRKTLKRINALIADIKRDPDGQGIGKPEVLRNNLAGLRSRRIDDEHRLIYAIEPSQITIIS
CRYHYQ

Nucleotide

• Length: 261 bp

>T21414 NC_007777:3018165-3018425 [Frankia casuarinae]
GTGAAGCTGACCTGGACCGACCATGCCTGGGACGACTACCTGTACTGGCAGAGCCAGGACCGCAAAACTCTCAAGCGCAT
CAACGCCCTGATCGCGGACATCAAACGCGATCCAGATGGGCAGGGCATCGGCAAACCGGAAGTGCTGCGCAACAACCTCG
CCGGGCTGCGCTCCCGGCGAATCGACGACGAGCACCGCCTGATCTATGCGATTGAGCCCAGCCAGATCACAATCATCTCC
TGCCGCTACCACTATCAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9361.53 Da
• Isoelectric Point: 4.7815

>AT21414 WP_011436953.1 NC_007777:3017911-3018168 [Frankia casuarinae]
MVAAISASEARKTLFPLLEQVNADHTPVEIVSKRGNAVLVSKEDWDAIVETNYLLRTPANARRLMESVEQWRSGGAVERS
PDPDG

Nucleotide

• Length: 258 bp

>AT21414 NC_007777:3017911-3018168 [Frankia casuarinae]
ATGGTCGCGGCGATCAGTGCCAGCGAGGCGCGCAAGACACTGTTCCCACTTCTCGAACAGGTCAACGCCGATCACACACC
GGTAGAGATCGTCTCGAAGAGGGGGAACGCGGTCCTGGTGAGCAAGGAGGACTGGGACGCGATCGTGGAGACCAACTACC
TGCTGCGCACGCCTGCCAACGCCCGCCGGCTGATGGAGAGCGTCGAGCAGTGGCGTTCAGGCGGCGCGGTCGAGCGTTCC
CCGGATCCTGACGGGTGA

Structures

Toxin

• AlphaFold DB: Q2J9X3

Antitoxin

• AlphaFold DB: Q2J9X4