Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 31558..32356 Replicon plasmid pIncFIB
Accession NZ_CP082122
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag K5P75_RS24270 Protein ID WP_000072677.1
Coordinates 31835..32356 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag K5P75_RS24265 Protein ID WP_001351987.1
Coordinates 31558..31827 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS24240 (K5P75_24240) 26985..28325 - 1341 WP_000137333.1 DnaB-like helicase C-terminal domain-containing protein -
K5P75_RS24245 (K5P75_24245) 28369..29109 - 741 WP_203243908.1 hypothetical protein -
K5P75_RS24250 (K5P75_24250) 29392..30159 + 768 WP_000342417.1 hypothetical protein -
K5P75_RS24255 (K5P75_24255) 30212..30565 - 354 WP_160378290.1 hypothetical protein -
K5P75_RS24260 (K5P75_24260) 30571..31239 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
K5P75_RS24265 (K5P75_24265) 31558..31827 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
K5P75_RS24270 (K5P75_24270) 31835..32356 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
K5P75_RS24275 (K5P75_24275) 32525..32776 - 252 WP_000901559.1 hypothetical protein -
K5P75_RS24280 (K5P75_24280) 32778..33470 - 693 WP_012640731.1 hypothetical protein -
K5P75_RS24285 (K5P75_24285) 33484..33807 - 324 WP_000064175.1 hypothetical protein -
K5P75_RS25555 34010..35401 - 1392 WP_261785286.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108374 108374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T214138 WP_000072677.1 NZ_CP082122:31835-32356 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T214138 NZ_CP109983:328161-328529 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT214138 WP_001351987.1 NZ_CP082122:31558-31827 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT214138 NZ_CP109983:327943-328164 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References