Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94169..94812 Replicon plasmid pIncFIA
Accession NZ_CP082121
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K5P75_RS24000 Protein ID WP_001044768.1
Coordinates 94396..94812 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K5P75_RS23995 Protein ID WP_001261287.1
Coordinates 94169..94399 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS23985 (89347) 89347..90480 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
K5P75_RS23990 (90743) 90743..93862 - 3120 WP_023909028.1 hypothetical protein -
K5P75_RS23995 (94169) 94169..94399 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5P75_RS24000 (94396) 94396..94812 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K5P75_RS24005 (94974) 94974..97112 - 2139 WP_000350635.1 AAA family ATPase -
K5P75_RS24010 (97577) 97577..98590 + 1014 WP_085674723.1 nucleotidyltransferase -
K5P75_RS24015 (98594) 98594..99526 + 933 WP_000991832.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / aac(6')-Ib-cr / dfrA17 / aadA5 / qacE / mph(A) / blaTEM-1B / aac(3)-IId / sul2 / aph(3'')-Ib / aph(6)-Id - 1..109135 109135
- flank IS/Tn - - 99663..100166 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T214137 WP_001044768.1 NZ_CP082121:94396-94812 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T214137 NZ_CP109978:c721-455 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT214137 WP_001261287.1 NZ_CP082121:94169-94399 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT214137 NZ_CP109978:c929-705 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References