Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4378746..4379252 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag K5P75_RS21230 Protein ID WP_085674725.1
Coordinates 4378746..4378928 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K5P75_RS21235 Protein ID WP_000398619.1
Coordinates 4378956..4379252 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS21220 (4375425) 4375425..4376882 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
K5P75_RS21225 (4377119) 4377119..4378636 + 1518 WP_001295063.1 lysine--tRNA ligase -
K5P75_RS21230 (4378746) 4378746..4378928 - 183 WP_085674725.1 type V toxin-antitoxin system toxin GhoT Toxin
K5P75_RS21235 (4378956) 4378956..4379252 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K5P75_RS21240 (4379479) 4379479..4379751 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K5P75_RS21245 (4379763) 4379763..4379993 - 231 WP_000371699.1 4Fe-4S mono-cluster protein YjdI -
K5P75_RS21250 (4380174) 4380174..4381805 + 1632 WP_222701176.1 sensor histidine kinase -
K5P75_RS21255 (4381802) 4381802..4382521 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6960.51 Da        Isoelectric Point: 8.6430

>T214132 WP_085674725.1 NZ_CP082120:c4378928-4378746 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLFYEL

Download         Length: 183 bp

>T214132 NZ_CP109974:32624-32935 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT214132 WP_000398619.1 NZ_CP082120:c4379252-4378956 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT214132 NZ_CP109974:32932-33351 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References