Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3541128..3541746 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K5P75_RS17200 Protein ID WP_001291435.1
Coordinates 3541528..3541746 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K5P75_RS17195 Protein ID WP_000344800.1
Coordinates 3541128..3541502 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS17185 (3536217) 3536217..3537410 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5P75_RS17190 (3537433) 3537433..3540582 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K5P75_RS17195 (3541128) 3541128..3541502 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K5P75_RS17200 (3541528) 3541528..3541746 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K5P75_RS17205 (3541918) 3541918..3542469 + 552 WP_000102564.1 maltose O-acetyltransferase -
K5P75_RS17210 (3542585) 3542585..3543055 + 471 WP_000136192.1 YlaC family protein -
K5P75_RS17215 (3543219) 3543219..3544769 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K5P75_RS17220 (3544811) 3544811..3545164 - 354 WP_000878141.1 DUF1428 family protein -
K5P75_RS17230 (3545543) 3545543..3545854 + 312 WP_000409911.1 MGMT family protein -
K5P75_RS17235 (3545885) 3545885..3546457 - 573 WP_000779839.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T214128 WP_001291435.1 NZ_CP082120:3541528-3541746 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T214128 NZ_CP109973:c4880694-4880410 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT214128 WP_000344800.1 NZ_CP082120:3541128-3541502 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT214128 NZ_CP109973:c4880926-4880684 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References