Detailed information of TA system TA214127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2493549..2494187
• Replicon: chromosome
• Accession: NZ_CP082120
• Organism: Escherichia coli strain PC-NDM34

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K5P75_RS12080
• Protein ID: WP_000813794.1
• Coordinates: 2494011..2494187 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K5P75_RS12075
• Protein ID: WP_001270286.1
• Coordinates: 2493549..2493965 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P75_RS12055 (2488701)	2488701..2489642	-	942	WP_001251315.1	ABC transporter permease	-
K5P75_RS12060 (2489643)	2489643..2490656	-	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
K5P75_RS12065 (2490674)	2490674..2491819	-	1146	WP_000047455.1	ABC transporter substrate-binding protein	-
K5P75_RS12070 (2492064)	2492064..2493470	-	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
K5P75_RS12075 (2493549)	2493549..2493965	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K5P75_RS12080 (2494011)	2494011..2494187	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K5P75_RS12085 (2494409)	2494409..2494639	+	231	WP_000494244.1	YncJ family protein	-
K5P75_RS12090 (2494731)	2494731..2496692	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K5P75_RS12095 (2496765)	2496765..2497301	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K5P75_RS12100 (2497354)	2497354..2498568	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2498608..2499873	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T214127 WP_000813794.1 NZ_CP082120:c2494187-2494011 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T214127 NZ_CP109973:c4776344-4775811 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT214127 WP_001270286.1 NZ_CP082120:c2493965-2493549 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT214127 NZ_CP109973:c4776621-4776355 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 6HPB