Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1869160..1869991 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K5P75_RS08945 Protein ID WP_000854814.1
Coordinates 1869160..1869534 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag K5P75_RS08950 Protein ID WP_001285585.1
Coordinates 1869623..1869991 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS08905 (1864556) 1864556..1865722 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
K5P75_RS08910 (1865841) 1865841..1866314 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
K5P75_RS08915 (1866512) 1866512..1867570 + 1059 WP_001200891.1 FUSC family protein -
K5P75_RS08920 (1867742) 1867742..1868071 + 330 WP_000450409.1 DUF496 family protein -
K5P75_RS25370 (1868172) 1868172..1868495 - 324 WP_225343796.1 EutP/PduV family microcompartment system protein -
K5P75_RS25375 (1868474) 1868474..1868554 + 81 WP_023441679.1 hypothetical protein -
K5P75_RS08935 (1868843) 1868843..1868923 - 81 Protein_1743 hypothetical protein -
K5P75_RS08940 (1868969) 1868969..1869163 - 195 WP_000988600.1 DUF5983 family protein -
K5P75_RS08945 (1869160) 1869160..1869534 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K5P75_RS08950 (1869623) 1869623..1869991 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K5P75_RS08955 (1870065) 1870065..1870286 - 222 WP_000692345.1 DUF987 domain-containing protein -
K5P75_RS08960 (1870349) 1870349..1870825 - 477 WP_001186775.1 RadC family protein -
K5P75_RS08965 (1870841) 1870841..1871320 - 480 WP_021525716.1 antirestriction protein -
K5P75_RS08970 (1871402) 1871402..1872223 - 822 WP_001234530.1 DUF932 domain-containing protein -
K5P75_RS08975 (1872444) 1872444..1872854 - 411 WP_124038838.1 hypothetical protein -
K5P75_RS08980 (1872870) 1872870..1873553 - 684 WP_000775500.1 hypothetical protein -
K5P75_RS08985 (1873689) 1873689..1874759 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T214118 WP_000854814.1 NZ_CP082120:c1869534-1869160 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T214118 NZ_CP109969:c262385-262104 [Agrobacterium salinitolerans]
ATGATCCGTTCCTTCAAAAACAAGCTGACCGCATCGATTGACGACGGATCTGTGAAGAAGGGCTTTCCCGCCGATCTGGT
ACGCCGCGCCCAGCAGCTGCTCACGATCCTGGATGCGGCGACCACGGTAGAAGACCTTCGCTCACCGCCCGGCAATCGTT
TGGAAAAGCTGTCCGGCGATCGTGAGGGCCAATATTCGATCCGGATCAACAAGCAGTGGCGCATCTGCTTCGTCTGGACG
GAAGCAGGACCGGAAAGCGTGGAAATAACCGACTATCACTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT214118 WP_001285585.1 NZ_CP082120:c1869991-1869623 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT214118 NZ_CP109969:c262048-261749 [Agrobacterium salinitolerans]
ATGACAATCAGCAAATCGCCTCCGATCCATCCGGGCGAAATCCTGCGGGAACTCTATCTTGAGCCGCTGGGGATGTCGGC
TTACGCTCTTGCAAGGCACCTCAATATTCCGCGCACCCGCATAGAACGGATTGCGGGAGAGAAACTTGGTATCACCGCCG
ACACCGCCTTGCGCCTTGCAAAATTTTTCAAGACCACACCGGAATATTGGCTGAACCTGCAGAACGCCTTTGACATTAAG
GCGGCGGCTGCGAGGCTGGAAGCAGACCTTGCCAATATCAAAGAACTGGAAGCGGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References