Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 915024..915678 Replicon chromosome
Accession NZ_CP082120
Organism Escherichia coli strain PC-NDM34

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K5P75_RS04510 Protein ID WP_000244772.1
Coordinates 915271..915678 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K5P75_RS04505 Protein ID WP_000354046.1
Coordinates 915024..915290 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P75_RS04480 (910312) 910312..911055 + 744 WP_000951940.1 SDR family oxidoreductase -
K5P75_RS04485 (911112) 911112..912545 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
K5P75_RS04490 (912590) 912590..912901 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K5P75_RS04495 (913065) 913065..913724 + 660 WP_000250274.1 hemolysin III family protein -
K5P75_RS04500 (913801) 913801..914781 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K5P75_RS04505 (915024) 915024..915290 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K5P75_RS04510 (915271) 915271..915678 + 408 WP_000244772.1 protein YgfX Toxin
K5P75_RS04515 (915718) 915718..916239 - 522 WP_001055874.1 flavodoxin FldB -
K5P75_RS04520 (916351) 916351..917247 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K5P75_RS04525 (917272) 917272..917982 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K5P75_RS04530 (917988) 917988..919721 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T214114 WP_000244772.1 NZ_CP082120:915271-915678 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T214114 NZ_CP109966:181875-182174 [Catenovulum sp. TS8]
ATGGCTGAAATAATATGGACTGAGCCTGCTTTAAATGATCTAAATGAAATTGCAGAATATATAGCTTTAAGTAATTTAAC
TGCGGCTAAAAATTTAACTCAAAAAGTATTTAATAAAATATCGAGGCTAGAGAATCACCCAGAATCAGGGAAGGTTCCTA
GTGAACTAAAAGGTTTTAGCTATAGAGAAGTTGTTGTTAACCCATGTCGGGTCTTTTATAAATTAGAAAATAATCAAGTT
TATATATTACATGTCATGCGACAAGAACGTGATCTTAGAAGATTTCTTCTACAGAATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT214114 WP_000354046.1 NZ_CP082120:915024-915290 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT214114 NZ_CP109966:181630-181887 [Catenovulum sp. TS8]
ATGAAAGTAGAACTAGTCACGACTCTAAAAAGGCAAGCCACTAAAATCTTAGCTGATCTTCATTCATCAAAAGAACCCGT
TTTAATTACTGAGCACGGACAACCATCTGCATATTTAGTTGATGTAGAGGATTATGAATTCATGCAACAACGAATGGAAA
TCCTTGAAGGTGTTGCAAGGGGCGAGCAAGCGATTAAAAATGGAAATACATTTTCGAATCAAGAAGCAAAAGAGAAAATG
AGTAAATGGCTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References