Detailed information of TA system TA214111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1200..1780
• Replicon: plasmid pKP19-2196-3
• Accession: NZ_CP082044
• Organism: Klebsiella pneumoniae strain KP19-2196

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K6165_RS29845
• Protein ID: WP_071177730.1
• Coordinates: 1200..1514 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K6165_RS29850
• Protein ID: WP_000093040.1
• Coordinates: 1502..1780 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6165_RS29835	403..582	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K6165_RS29840	608..1036	-	429	WP_001140599.1	hypothetical protein	-
K6165_RS29845	1200..1514	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6165_RS29850	1502..1780	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K6165_RS30215	1955..2320	-	366	WP_072354022.1	TonB family protein	-
K6165_RS29860	2317..2688	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
K6165_RS29865	2962..3207	-	246	WP_032440458.1	hypothetical protein	-
K6165_RS29870	3534..5219	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
K6165_RS29875	5229..5486	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
K6165_RS29880	5571..5720	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10049	10049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T214111 WP_071177730.1 NZ_CP082044:c1514-1200 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T214111 NZ_CP109965:c2612637-2611747 [Catenovulum sp. TS8]
ATGTTAAAACAACAAATACGCCAAATAATCCAAGCTAATCCAGAATATGCCGCGATTACTCCAGTAATAGAAAAAGAAAT
ACTGCATCATGACATTATGAATGTACTTATTAAACAAGGTGCAATGCAGACCTTGAGCTTTATTGGCGGTACTTCATTAA
GAATGTGTTACAACAGCGCCAGACTTTCCGAAGACCTTGACTTTAATGGTGGACATGATTTTAAACCGTCTGATTTTGAT
GGTTTAGAAATTGAAATCCAAAAGTACATTCAAAAAAAATATGAGACCGATGTTTGGGTTAACAAACCAGCTGAAGATAA
TCAAGGTAATACTGCATCGTGGAAAATAAGTATTGAAAAAGAAGCTAACCGGCCTGATTTACCTAGGCAAAAAATGCATA
TAGATGTTTGTGCTATCCCTTCTTTTGATATTGAAAAACGGCCATTGCTTAATCACTATAATATTGTGGTGCCAACAGAA
GGCATACTTGTTCCAGTACAATCGCTACAAGAGATTCTAGCAGATAAATTAATCGCATTAGCCTATCGTTCAAGACGAAT
TAAACCCAGAGATATTTGGGATATTGTTTGGATTAAACAGCGCGGAATGAGTTTATCAAGAGAGCTAGTTGAAAAAAAAC
TAGCCGCCAGAAACAAACCATTGGATGATTTTCGTCAAGCATTAAGCACTCAACTAAATAAATTGATGCAAAATACGGAA
GTAAAAGATGATTTTAATATGGAAATGAGCCGATTTATCCCTCAGAAAATTAAAGAAAGAACTTTAGATAACCCTGAGTA
TTGGGCTTATGTGCAAGCAGAGGTAAATGAGATAGCTTCCGCTCTTCTGCGTGATAACGCATCAAGAAATAAGTTTGATA
TGGGGTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT214111 WP_000093040.1 NZ_CP082044:c1780-1502 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT214111 NZ_CP109965:c2613176-2612652 [Catenovulum sp. TS8]
ATGACAAAAACTGACAAACTTCTCTCCGTTTTTGCTAGCTCAGTTAAAACAGGTGGCGGTATTCATACTGCGTCAGAGTT
GGCATTTATGCTCGGGGAACCTTGCAATACAGCATTTTTAAAATTGTTGGCTGATGGTGTTAAAAAAGAAAAATTTAGAA
GAGTTGCGAAAGGACTATATGAAAGCACACTTACACCACCAGAGCCTGAGAGCGCTATTTATAAAATAATCACTAAATTA
CGTAGCAATGTTCTTAATTATATTAGTTTGGAGAGTCAGTTAAGTCATACCGGTGATATTTCACAAGTGGTGATGGATAG
ATTGACTGTTATCACCAAGGGACGAAGTGGTACGTTTGCAACACCATATGGATTGATTGAATTTACTCATACTAAAAAAA
CCATTGAACAAATTGCCCCTAATTTATATTTTGATCCCGATATTAAAATGTATCGAGCAACAACAAAACAAGCGATAGAA
GACCTTAAACATTGTAACCGCAATTTGCATATGCTGAACCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1