Detailed information of TA system TA021408

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1731975..1732569
• Replicon: chromosome
• Accession: NC_007761
• Organism: Rhizobium etli CFN 42

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RHE_RS08415
• Protein ID: WP_042118257.1
• Coordinates: 1731975..1732364 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RHE_RS08420
• Protein ID: WP_042118259.1
• Coordinates: 1732348..1732569 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHE_RS08395	1727217..1727837	-	621	WP_011424955.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
RHE_RS08400	1727884..1729173	-	1290	WP_011424956.1	dihydroorotase	-
RHE_RS08405	1729170..1730126	-	957	WP_011424957.1	aspartate carbamoyltransferase catalytic subunit	-
RHE_RS08410	1730305..1731963	+	1659	WP_011424958.1	acyl-CoA dehydrogenase family protein	-
RHE_RS08415	1731975..1732364	-	390	WP_042118257.1	PIN domain nuclease	Toxin
RHE_RS08420	1732348..1732569	-	222	WP_042118259.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RHE_RS08425	1732716..1735601	-	2886	WP_011424960.1	Rne/Rng family ribonuclease	-
RHE_RS08430	1736224..1737483	+	1260	WP_042118261.1	N-acetylmuramoyl-L-alanine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14719.02 Da
• Isoelectric Point: 6.6425

>T21408 WP_042118257.1 NC_007761:c1732364-1731975 [Rhizobium etli CFN 42]
VIVVDSSVWIAHLRGIENAARRRLQNIEDPLEILVGDLILMEVLQGARDDRHATTIEANLRQFTIRPMLDDSLAVRAARN
YRLLRERGITMRKTIDVIIGTFCIQEGHSLLHDDRDFDPMAQHLGLKIA

Nucleotide

• Length: 390 bp

>T21408 NC_007761:c1732364-1731975 [Rhizobium etli CFN 42]
GTGATCGTCGTCGATAGTTCGGTCTGGATTGCGCATCTACGTGGGATAGAAAACGCCGCTAGACGGCGTCTGCAGAATAT
CGAAGATCCGCTGGAGATTCTTGTTGGCGATCTTATTCTTATGGAGGTCCTCCAAGGCGCGCGCGATGACCGTCATGCAA
CCACCATCGAAGCGAATTTGCGCCAATTCACGATCCGTCCGATGCTTGACGACAGCCTCGCCGTTAGGGCAGCCCGCAAT
TATCGCCTGCTGCGGGAACGCGGTATCACAATGCGCAAGACCATCGACGTAATCATTGGCACATTCTGCATTCAAGAAGG
CCACTCGCTCTTGCACGATGATAGGGATTTCGATCCCATGGCCCAGCATCTCGGTCTCAAGATCGCCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8243.31 Da
• Isoelectric Point: 10.4369

>AT21408 WP_042118259.1 NC_007761:c1732569-1732348 [Rhizobium etli CFN 42]
MRTNIDIDDALLHAAMTATGLATKKATIEQALRSLVDRHRRKNAIANLSGIGWEGDLEEMRRDRPEGTSDRRR

Nucleotide

• Length: 222 bp

>AT21408 NC_007761:c1732569-1732348 [Rhizobium etli CFN 42]
ATGCGGACGAATATAGATATCGATGATGCCTTGCTCCACGCAGCGATGACCGCCACGGGGTTGGCGACGAAAAAAGCTAC
CATTGAACAGGCCCTCCGAAGCCTCGTCGACAGGCACCGCAGAAAAAATGCGATCGCAAATCTCTCTGGAATCGGCTGGG
AGGGTGATCTGGAAGAGATGCGCCGCGATCGACCGGAAGGAACCAGTGATCGTCGTCGATAG