Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5497376..5498001 Replicon chromosome
Accession NZ_CP082037
Organism Klebsiella pneumoniae strain KP18-2172

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6161_RS27140 Protein ID WP_002882817.1
Coordinates 5497376..5497759 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6161_RS27145 Protein ID WP_004150355.1
Coordinates 5497759..5498001 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6161_RS27125 5494742..5495644 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6161_RS27130 5495641..5496276 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6161_RS27135 5496273..5497202 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6161_RS27140 5497376..5497759 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6161_RS27145 5497759..5498001 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6161_RS27150 5498206..5499123 + 918 WP_002882812.1 alpha/beta hydrolase -
K6161_RS27155 5499137..5500078 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6161_RS27160 5500123..5500560 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6161_RS27165 5500557..5501417 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6161_RS27170 5501411..5502010 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214077 WP_002882817.1 NZ_CP082037:c5497759-5497376 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214077 NZ_CP109953:2340959-2341177 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214077 WP_004150355.1 NZ_CP082037:c5498001-5497759 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214077 NZ_CP109953:2340559-2340933 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References