Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 5076494..5077164 Replicon chromosome
Accession NZ_CP082037
Organism Klebsiella pneumoniae strain KP18-2172

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag K6161_RS25110 Protein ID WP_004213072.1
Coordinates 5076494..5076937 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag K6161_RS25115 Protein ID WP_004213073.1
Coordinates 5076934..5077164 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6161_RS25075 5071915..5072181 + 267 WP_147021650.1 carboxymuconolactone decarboxylase family protein -
K6161_RS25080 5072244..5072735 + 492 WP_011251327.1 DM13 domain-containing protein -
K6161_RS25085 5072784..5073704 + 921 WP_004213934.1 DUF1471 domain-containing protein -
K6161_RS25090 5073795..5074198 + 404 Protein_4923 GAF domain-containing protein -
K6161_RS25095 5074716..5075350 - 635 Protein_4924 mucoid phenotype regulator RmpA2 -
K6161_RS25100 5075767..5076071 + 305 Protein_4925 transposase -
K6161_RS28770 5076094..5076345 - 252 WP_186987481.1 hypothetical protein -
K6161_RS25110 5076494..5076937 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
K6161_RS25115 5076934..5077164 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6161_RS25120 5077772..5078905 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
K6161_RS25125 5078921..5079214 + 294 WP_004213076.1 hypothetical protein -
K6161_RS25130 5079204..5079410 - 207 WP_004213077.1 hypothetical protein -
K6161_RS25135 5079762..5080052 + 291 WP_004213078.1 hypothetical protein -
K6161_RS25140 5080042..5080941 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187 iucA / iucB / iucC / iucD / iutA / rmpA 4982432..5178366 195934


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T214075 WP_004213072.1 NZ_CP082037:c5076937-5076494 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T214075 NZ_CP109953:1528158-1528265 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT214075 WP_004213073.1 NZ_CP082037:c5077164-5076934 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT214075 NZ_CP109953:c1528111-1528045 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References