Detailed information of TA system TA214057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2105..2579
• Replicon: plasmid pKP18-2172-3
• Accession: NZ_CP082036
• Organism: Klebsiella pneumoniae strain KP18-2172

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K6161_RS00030
• Protein ID: WP_004197762.1
• Coordinates: 2313..2579 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K6161_RS00025
• Protein ID: WP_000879771.1
• Coordinates: 2105..2329 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6161_RS28830	241..675	+	435	Protein_1	relaxase/mobilization nuclease domain-containing protein	-
K6161_RS00015	710..1219	+	510	WP_032433972.1	MbeB family mobilization protein	-
K6161_RS00020	1226..1438	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K6161_RS28835	1814..1942	-	129	WP_000523570.1	hypothetical protein	-
K6161_RS00025	2105..2329	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K6161_RS00030	2313..2579	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6161_RS00035	2649..2999	-	351	WP_004197756.1	hypothetical protein	-
K6161_RS00040	3065..3610	-	546	WP_023317533.1	hypothetical protein	-
K6161_RS00045	3636..3962	-	327	WP_050516389.1	hypothetical protein	-
K6161_RS00050	5118..5294	+	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T214057 WP_004197762.1 NZ_CP082036:2313-2579 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T214057 NZ_CP109948:4152636-4152854 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT214057 WP_000879771.1 NZ_CP082036:2105-2329 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT214057 NZ_CP109948:4152235-4152609 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: S1EJF5