Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61822..62465 Replicon plasmid pKP18-2138-KPC2
Accession NZ_CP082034
Organism Klebsiella pneumoniae strain KP18-2138

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6163_RS28355 Protein ID WP_001044770.1
Coordinates 61822..62238 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6163_RS28360 Protein ID WP_001261282.1
Coordinates 62235..62465 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6163_RS28335 (57925) 57925..58197 - 273 Protein_80 transposase -
K6163_RS28345 (59179) 59179..60201 - 1023 WP_000361404.1 helicase UvrD -
K6163_RS28350 (60186) 60186..61748 - 1563 WP_004206609.1 AAA family ATPase -
K6163_RS28355 (61822) 61822..62238 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6163_RS28360 (62235) 62235..62465 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6163_RS28365 (62422) 62422..62883 + 462 WP_014343465.1 hypothetical protein -
K6163_RS28370 (63044) 63044..63988 + 945 WP_011977810.1 hypothetical protein -
K6163_RS28375 (64025) 64025..64417 + 393 WP_011977811.1 hypothetical protein -
K6163_RS28380 (64475) 64475..64996 + 522 WP_013214008.1 hypothetical protein -
K6163_RS28385 (65042) 65042..65245 + 204 WP_011977813.1 hypothetical protein -
K6163_RS28390 (65275) 65275..66279 + 1005 WP_011977814.1 hypothetical protein -
K6163_RS28395 (66463) 66463..67242 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaSHV-12 / blaKPC-2 - 1..115559 115559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T214054 WP_001044770.1 NZ_CP082034:c62238-61822 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T214054 NZ_CP109948:2168879-2169055 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGTATCAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT214054 WP_001261282.1 NZ_CP082034:c62465-62235 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT214054 NZ_CP109948:2169103-2169510 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCTTGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGTACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References