Detailed information of TA system TA214048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5148808..5149334
• Replicon: chromosome
• Accession: NZ_CP082032
• Organism: Klebsiella pneumoniae strain KP18-2138

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: K6163_RS25500
• Protein ID: WP_000323025.1
• Coordinates: 5148808..5149095 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: K6163_RS25505
• Protein ID: WP_000534858.1
• Coordinates: 5149095..5149334 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6163_RS25470	5143854..5144087	-	234	WP_004181905.1	hypothetical protein	-
K6163_RS25475	5144166..5144513	-	348	WP_004181904.1	hypothetical protein	-
K6163_RS25480	5144669..5145736	-	1068	WP_004181903.1	hypothetical protein	-
K6163_RS25485	5146426..5146803	+	378	WP_004181901.1	hypothetical protein	-
K6163_RS25490	5147001..5147975	-	975	WP_004181899.1	hypothetical protein	-
K6163_RS25495	5148578..5148736	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
K6163_RS25500	5148808..5149095	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
K6163_RS25505	5149095..5149334	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
K6163_RS29000	5149359..5149463	+	105	Protein_5020	hypothetical protein	-
K6163_RS25510	5149597..5149920	-	324	WP_224948387.1	cation transporter dimerization domain-containing protein	-
K6163_RS25515	5150086..5151254	+	1169	WP_085929673.1	IS3-like element ISEc52 family transposase	-
K6163_RS25520	5151264..5151575	-	312	Protein_5023	transposase	-
K6163_RS25525	5151996..5153336	-	1341	WP_000589001.1	ISNCY family transposase	-
K6163_RS25530	5153568..5154272	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187	rmpA / iutA / iucD / iucC / iucB / iucA	5058728..5216636	157908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T214048 WP_000323025.1 NZ_CP082032:c5149095-5148808 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T214048 NZ_CP109947:c1184151-1183882 [Pectobacterium cacticida]
ATGAAACTGCTATGGACAGAGAATGCTTGGCAGGACTATTTATACTGGCAGGAAAATGATCCCGGTATGGTCACAAAAAT
CAACGATCTGATTAAGGATACCAAACGGTCGCCCTTCAAAGGATTAGGGAAACCAGAACCCCTTAAAGGTGACATCTCAG
GCTTTTGGTCGCGACGGATATCCGGTGAACATCGTTTTGTCTACCGTGTGTCGGGAAAAGGCGAGGCTCAGCAACTGGAT
GTTGTTCAATGCCGGTTCCATTATAAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT214048 WP_000534858.1 NZ_CP082032:c5149334-5149095 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT214048 NZ_CP109947:c1184404-1184153 [Pectobacterium cacticida]
ATGGCACACGTTCGTTTGACCGAATTCCGCCAGAACATTGCCGCCCATTTTGATGAAGTTATCTCATCAAGAGCGCCGTT
GCTGGTTACACGCCAAGGGTCAGAAGCAATGGTCATGCTGGCTGAAGGTGAATACGAATCCATTTTGGAAACGCTGCACT
TGCTTTCAACTCCGGCAAACACGGATCGGCTACGTGAGAGCATTAAGCAGCTTGGTGACGGGAAAGTGGTAGAGAGGGAT
CCGACTCTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8