Detailed information of TA system TA214047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5072119..5072789
• Replicon: chromosome
• Accession: NZ_CP082032
• Organism: Klebsiella pneumoniae strain KP18-2138

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6163_RS25105
• Protein ID: WP_004213072.1
• Coordinates: 5072346..5072789 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6163_RS25100
• Protein ID: WP_004213073.1
• Coordinates: 5072119..5072349 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6163_RS25065	5067163..5067948	-	786	WP_002885391.1	DsbA family protein	-
K6163_RS25070	5068174..5068857	+	684	WP_004199298.1	TerC family protein	-
K6163_RS25075	5068814..5068927	-	114	WP_002885341.1	hypothetical protein	-
K6163_RS25080	5069003..5069920	+	918	WP_002885338.1	curved DNA-binding protein	-
K6163_RS25085	5069920..5070225	+	306	WP_002885324.1	chaperone modulator CbpM	-
K6163_RS25090	5070394..5070819	+	426	Protein_4936	IS3 family transposase	-
K6163_RS25095	5070884..5071588	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6163_RS25100	5072119..5072349	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6163_RS25105	5072346..5072789	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6163_RS28990	5072938..5073189	+	252	WP_186987481.1	hypothetical protein	-
K6163_RS25115	5073212..5073516	-	305	Protein_4941	transposase	-
K6163_RS25120	5073933..5074567	+	635	Protein_4942	mucoid phenotype regulator RmpA2	-
K6163_RS25125	5075085..5075488	-	404	Protein_4943	GAF domain-containing protein	-
K6163_RS25130	5075579..5076499	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6163_RS25135	5076548..5077039	-	492	WP_011251327.1	DM13 domain-containing protein	-
K6163_RS25140	5077102..5077368	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187	rmpA / iutA / iucD / iucC / iucB / iucA	5058728..5216636	157908
	-	flank	IS/Tn	-	-	5070884..5071588	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T214047 WP_004213072.1 NZ_CP082032:5072346-5072789 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T214047 NZ_CP109947:c1176425-1176027 [Pectobacterium cacticida]
ATCAGGATTTTCAAGTCAGCACTGATCCGTAAGCAATTGAGCCAACAAGAGCTTGATGATTTGGCGGCGGATTTTCTTTC
CTACAAAAAGGATGGCGTGTTGCCAGATACCTTTGGTCGTGATGCACCCTATGATGATGACCGCACCTATCCCTTGGTAA
AAGAAGAACAAGTGGCCCACATTCATCTTGCCGATGCGGATGCCCCTTTCCCGAAGTTTTTACGACAGTTCAAACGAACC
AGCGATCAGGCGCATTTGGTATACTGCCAAGGTGCGATGGATCCCGATGCCTACCTGCTGATTATCATCCTTAAACCCGA
AGCACATAAAATGGCACGCAACAATAACCATATGCATAAGATCGGGATGATGGCAGAAGCGTTCAGGATGAAGCATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT214047 WP_004213073.1 NZ_CP082032:5072119-5072349 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT214047 NZ_CP109947:c1176721-1176428 [Pectobacterium cacticida]
ATGGAACGCATCCTCGCAGAAAAATCTATCAACATCACCGAGTTAAGAAAGAACCCGGCGAAATATTTTATTGATGAACC
GGTTGCCGTTCTCTCCAACAACCGTCCCGCTGGCTACATGGTCAGTGCTAAGGTGTTTGAAGAGCTGATCGATCTGTTGG
AAGAGAAGCAAGGCAGGGTTCATACCGCTGCGCGGTTCAGGCCATCTGCTGAACGTTTAAGCGATATTGCGGATAGCGGG
CAGAAACTGCTGCAAAACGCCTCCGACAAGGATCTGACCGAGTTCACGGAATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620