Detailed information of TA system TA214030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 188501..189144
• Replicon: plasmid pKP18-2113-1
• Accession: NZ_CP082031
• Organism: Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K6164_RS28090
• Protein ID: WP_014386165.1
• Coordinates: 188728..189144 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: K6164_RS28085
• Protein ID: WP_032408893.1
• Coordinates: 188501..188731 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6164_RS28060	183574..184557	-	984	WP_001471744.1	NAD(P)H-quinone oxidoreductase	-
K6164_RS28065	184576..185724	-	1149	WP_094965598.1	ABC transporter ATP-binding protein	-
K6164_RS28070	185895..187052	-	1158	WP_032408895.1	acetylornithine deacetylase	-
K6164_RS28075	187068..187742	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
K6164_RS28080	187747..188181	-	435	WP_000103648.1	RidA family protein	-
K6164_RS28085	188501..188731	+	231	WP_032408893.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6164_RS28090	188728..189144	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
K6164_RS28095	189467..190435	+	969	WP_074422984.1	IS5-like element IS903B family transposase	-
K6164_RS28100	190615..190989	-	375	WP_032408891.1	hypothetical protein	-
K6164_RS28105	191045..191371	-	327	WP_004152639.1	hypothetical protein	-
K6164_RS28110	191368..192096	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
K6164_RS28115	192093..192524	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..212588	212588
	-	flank	IS/Tn	-	-	189512..190435	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T214030 WP_014386165.1 NZ_CP082031:188728-189144 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T214030 NZ_CP109941:c1894976-1894647 [Streptococcus suis]
ATGGAATTTGAGTCTTATTCAGTGATACTTGCTCCAGCAGTGGAGAAGGAATTGGCAGTTATTTACGCCTATTTTTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTAGAGAGTTTGCAAATTTTTCCTGAAC
GTGGTTTTAACGCCGATAACCGTTTTGGTAAGCAGATAGACCCGCCTCATCTAACCCGAGGCTATGTCGTTGGAAAAGAC
TACATCGCCCTCTATCGCGTAGTGAAAAATGAAGTTCGAGTTGGTCATCTTTTTGCGACCAAGAGTGATTATGTGAAGTT
GTTGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT214030 WP_032408893.1 NZ_CP082031:188501-188731 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT214030 NZ_CP109941:c1895253-1894966 [Streptococcus suis]
TTGATAACTATAAATAAAGATGCCCAAGTAAATTTTAAAACTGATTCCAGACTTTTAGAAAAAGCCAAGGAAGTATTTGC
TATGAACCAGCTGGATTTGACTGCTGGTTTTAACCTCTTCTTGCAAAATATCGCTGTAAAAAATGAGTTGCCAATTCTGA
CAGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAGTTACAGAAGGAAATCCAGGAAAACCAACAGGCGATTGAGGCA
GGTCACGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA