Detailed information of TA system TA214029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 164605..165350
• Replicon: plasmid pKP18-2113-1
• Accession: NZ_CP082031
• Organism: Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: K6164_RS27960
• Protein ID: WP_032408901.1
• Coordinates: 164859..165350 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: K6164_RS27955
• Protein ID: WP_014386183.1
• Coordinates: 164605..164871 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6164_RS27905	160161..160574	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
K6164_RS27910	160575..160853	-	279	WP_004152721.1	helix-turn-helix transcriptional regulator	-
K6164_RS27915	160843..161163	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K6164_RS27920	161244..161468	-	225	WP_014386189.1	hypothetical protein	-
K6164_RS27925	161479..161691	-	213	WP_014386188.1	hypothetical protein	-
K6164_RS27930	161753..162079	-	327	WP_014386187.1	hypothetical protein	-
K6164_RS27935	162712..163062	-	351	WP_014386186.1	hypothetical protein	-
K6164_RS27940	163059..163331	-	273	WP_032408902.1	hypothetical protein	-
K6164_RS27945	163521..164006	+	486	WP_014386185.1	hypothetical protein	-
K6164_RS27950	164250..164408	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
K6164_RS27955	164605..164871	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
K6164_RS27960	164859..165350	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
K6164_RS27965	165791..166042	-	252	WP_032408900.1	aldo/keto reductase	-
K6164_RS27970	166239..167831	-	1593	Protein_184	IS66 family transposase	-
K6164_RS27975	167862..168212	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
K6164_RS27980	168209..168649	-	441	WP_014386179.1	transposase	-
K6164_RS27985	168911..169666	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..212588	212588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T214029 WP_032408901.1 NZ_CP082031:164859-165350 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T214029 NZ_CP109941:c1893847-1893590 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT214029 WP_014386183.1 NZ_CP082031:164605-164871 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT214029 NZ_CP109941:c1894109-1893849 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6