Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53668..54404 Replicon plasmid pKP18-2113-1
Accession NZ_CP082031
Organism Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K6164_RS27340 Protein ID WP_003026803.1
Coordinates 53922..54404 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K6164_RS27335 Protein ID WP_003026799.1
Coordinates 53668..53934 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6164_RS27290 49730..50092 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
K6164_RS27295 50142..50492 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K6164_RS27300 50850..51119 + 270 WP_004152102.1 hypothetical protein -
K6164_RS27305 51107..51682 + 576 WP_004152103.1 hypothetical protein -
K6164_RS27310 51713..52207 + 495 WP_004152104.1 DNA-binding protein -
K6164_RS27315 52251..52619 + 369 WP_004152105.1 hypothetical protein -
K6164_RS27320 52653..52856 + 204 WP_004152106.1 HHA domain-containing protein -
K6164_RS27325 52905..53162 + 258 WP_004152107.1 hypothetical protein -
K6164_RS27330 53238..53492 + 255 WP_004152108.1 hypothetical protein -
K6164_RS27335 53668..53934 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K6164_RS27340 53922..54404 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K6164_RS27345 54605..56008 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
K6164_RS27350 56037..56669 - 633 WP_001567369.1 hypothetical protein -
K6164_RS27355 56899..58245 + 1347 WP_020314316.1 ISNCY family transposase -
K6164_RS28455 58406..58537 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A) - 1..212588 212588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T214028 WP_003026803.1 NZ_CP082031:53922-54404 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T214028 NZ_CP109941:c1417754-1417494 [Streptococcus suis]
ATGTATCATTTAGAGTATTCTAAAAAGGCTCAAAAGCAAATTATGAAATTAGATAGGCAAATTCAGAGGCTGTTGTTTGC
TTGGATTGATAAACACTTAGAAGGGACTGATAATCCACGCGCCAATGGTAAAGGTTTGACAGGCAATCATGCTAACGAGT
GGCGTTATCGTATCGGAGATTACCGACTGATTTGCGATATTCAAGATGATAAGTTGGTTATTTTAGCACTTGAGTTTGGA
CATCGTAAAGATGTCTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT214028 WP_003026799.1 NZ_CP082031:53668-53934 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT214028 NZ_CP109941:c1417977-1417756 [Streptococcus suis]
ATGAGTACAGTGACAATCAGATTAAATCAAGAAGAAGAAGTATTCTTTAAAAGCTATGCACAATTAACAGGTCAAAGTCT
TTCTAGCCTTTTTAAGAAAGCTTTGGAGCGTGATATTGAAGATGAGTATGATTTAAAAATCTATCATCAAGCTTATGATG
AGTACAAAGCAGATCCAGAAACAATTAGTCATGCTGATTTTAAGAAAGAGCTAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References