Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5872..6397 Replicon plasmid pKP18-2113-KPC2
Accession NZ_CP082030
Organism Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag K6164_RS26455 Protein ID WP_013023785.1
Coordinates 5872..6177 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag K6164_RS26460 Protein ID WP_001568025.1
Coordinates 6179..6397 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6164_RS26425 (1551) 1551..2177 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
K6164_RS28365 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
K6164_RS26435 (2948) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
K6164_RS26440 (3940) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
K6164_RS26445 (4967) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
K6164_RS26450 (5308) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
K6164_RS26455 (5872) 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
K6164_RS26460 (6179) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K6164_RS26465 (6567) 6567..7028 - 462 WP_160880186.1 hypothetical protein -
K6164_RS26470 (6985) 6985..7215 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
K6164_RS26475 (7212) 7212..7628 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
K6164_RS26480 (7702) 7702..9264 + 1563 WP_004206609.1 AAA family ATPase -
K6164_RS26485 (9249) 9249..10271 + 1023 WP_000361404.1 helicase UvrD -
K6164_RS26490 (10527) 10527..11224 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..105927 105927


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T214026 WP_013023785.1 NZ_CP082030:c6177-5872 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T214026 NZ_CP109941:602963-603232 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT214026 WP_001568025.1 NZ_CP082030:c6397-6179 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT214026 NZ_CP109941:602746-602973 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGCCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References