Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5406377..5407002 Replicon chromosome
Accession NZ_CP082029
Organism Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6164_RS26055 Protein ID WP_002882817.1
Coordinates 5406377..5406760 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6164_RS26060 Protein ID WP_004150355.1
Coordinates 5406760..5407002 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6164_RS26040 (5403743) 5403743..5404645 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6164_RS26045 (5404642) 5404642..5405277 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6164_RS26050 (5405274) 5405274..5406203 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6164_RS26055 (5406377) 5406377..5406760 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6164_RS26060 (5406760) 5406760..5407002 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6164_RS26065 (5407207) 5407207..5408124 + 918 WP_004181612.1 alpha/beta hydrolase -
K6164_RS26070 (5408138) 5408138..5409079 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6164_RS26075 (5409124) 5409124..5409561 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6164_RS26080 (5409558) 5409558..5410418 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6164_RS26085 (5410412) 5410412..5411011 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T214025 WP_002882817.1 NZ_CP082029:c5406760-5406377 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T214025 NZ_CP109940:c2109083-2108826 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT214025 WP_004150355.1 NZ_CP082029:c5407002-5406760 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT214025 NZ_CP109940:c2109345-2109085 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References