Detailed information of TA system TA214007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 787957..788627
• Replicon: chromosome
• Accession: NZ_CP082029
• Organism: Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6164_RS03975
• Protein ID: WP_004213072.1
• Coordinates: 788184..788627 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6164_RS03970
• Protein ID: WP_004213073.1
• Coordinates: 787957..788187 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6164_RS03945 (784180)	784180..785079	-	900	WP_004225022.1	nucleotide-binding protein	-
K6164_RS03950 (785069)	785069..785359	-	291	WP_004213078.1	hypothetical protein	-
K6164_RS03955 (785711)	785711..785917	+	207	WP_004213077.1	hypothetical protein	-
K6164_RS03960 (785907)	785907..786200	-	294	WP_119062594.1	korC	-
K6164_RS03965 (786216)	786216..787349	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6164_RS03970 (787957)	787957..788187	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6164_RS03975 (788184)	788184..788627	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6164_RS28245 (788776)	788776..789027	+	252	WP_186987481.1	hypothetical protein	-
K6164_RS03985 (789050)	789050..789354	-	305	Protein_774	transposase	-
K6164_RS03990 (789771)	789771..790405	+	635	Protein_775	mucoid phenotype regulator RmpA2	-
K6164_RS03995 (790923)	790923..791326	-	404	Protein_776	GAF domain-containing protein	-
K6164_RS04000 (791417)	791417..792337	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6164_RS04005 (792386)	792386..792877	-	492	WP_011251327.1	DM13 domain-containing protein	-
K6164_RS04010 (792940)	792940..793206	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aac(3)-IId / blaSHV-187 / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	fimA / fimI / fimC / fimD / fimD / fimF / fimG / rmpA / iutA / iucD / iucC / iucB / iucA	579774..844483	264709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T214007 WP_004213072.1 NZ_CP082029:788184-788627 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T214007 NZ_CP109933:2466909-2467175 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT214007 WP_004213073.1 NZ_CP082029:787957-788187 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT214007 NZ_CP109933:2466658-2466909 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620