Detailed information of TA system TA214004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4871..5451
• Replicon: plasmid pKP18-2113-3
• Accession: NZ_CP082028
• Organism: Klebsiella pneumoniae strain KP18-2113

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K6164_RS00030
• Protein ID: WP_071177730.1
• Coordinates: 4871..5185 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K6164_RS00035
• Protein ID: WP_000093040.1
• Coordinates: 5173..5451 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6164_RS00005	815..2779	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
K6164_RS00010	2779..3510	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
K6164_RS00015	3517..4047	+	531	WP_071177729.1	hypothetical protein	-
K6164_RS00020	4074..4253	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K6164_RS00025	4279..4707	-	429	WP_001140599.1	hypothetical protein	-
K6164_RS00030	4871..5185	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6164_RS00035	5173..5451	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K6164_RS28215	5626..5991	-	366	WP_072354022.1	TonB family protein	-
K6164_RS00045	5988..6359	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
K6164_RS00050	6633..6878	-	246	WP_032440458.1	hypothetical protein	-
K6164_RS00055	7522..9009	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11969	11969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T214004 WP_071177730.1 NZ_CP082028:c5185-4871 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T214004 NZ_CP109933:c1753389-1753144 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT214004 WP_000093040.1 NZ_CP082028:c5451-5173 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT214004 NZ_CP109933:1752810-1753175 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTGGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1