Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 136247..136899 Replicon plasmid pKP18-2073-1
Accession NZ_CP082026
Organism Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag K6166_RS29430 Protein ID WP_017901321.1
Coordinates 136247..136672 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag K6166_RS29435 Protein ID WP_001261275.1
Coordinates 136669..136899 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6166_RS29400 131956..132072 + 117 Protein_138 transposase -
K6166_RS29405 132197..132367 - 171 Protein_139 LysR family transcriptional regulator -
K6166_RS30270 132378..132605 + 228 Protein_140 IS3 family transposase -
K6166_RS29415 132697..134253 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
K6166_RS30275 134440..134613 - 174 Protein_142 nuclease -
K6166_RS29420 134666..135202 - 537 Protein_143 integrase core domain-containing protein -
K6166_RS29425 135261..136229 - 969 WP_102097429.1 IS5 family transposase -
K6166_RS29430 136247..136672 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
K6166_RS29435 136669..136899 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6166_RS29440 137152..139728 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-187 - 1..237812 237812
- flank IS/Tn - - 135261..136229 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T214003 WP_017901321.1 NZ_CP082026:c136672-136247 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T214003 NZ_CP109933:c1744724-1744473 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT214003 WP_001261275.1 NZ_CP082026:c136899-136669 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT214003 NZ_CP109933:c1744483-1744310 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References