Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 66148..67058 Replicon plasmid pKP18-2073-1
Accession NZ_CP082026
Organism Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag K6166_RS29075 Protein ID WP_004144067.1
Coordinates 66588..67058 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag K6166_RS29070 Protein ID WP_023287184.1
Coordinates 66148..66591 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6166_RS29050 61564..62313 + 750 Protein_67 IS5 family transposase -
K6166_RS29055 62342..63310 + 969 WP_000654811.1 IS5 family transposase -
K6166_RS29060 63506..64852 + 1347 WP_227532884.1 Mov34/MPN/PAD-1 family protein -
K6166_RS29065 64905..66047 + 1143 Protein_70 translesion error-prone DNA polymerase V subunit UmuC -
K6166_RS29070 66148..66591 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
K6166_RS29075 66588..67058 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
K6166_RS29080 67145..68140 + 996 WP_077254794.1 IS5 family transposase -
K6166_RS29085 68142..69110 + 969 WP_072096747.1 IS5 family transposase -
K6166_RS29090 69299..69559 - 261 WP_017901409.1 hypothetical protein -
K6166_RS29095 69864..70832 + 969 WP_074423483.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-187 - 1..237812 237812
- inside IScluster/Tn - - 59420..70832 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T214001 WP_004144067.1 NZ_CP082026:66588-67058 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T214001 NZ_CP109933:1207270-1207458 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT214001 WP_023287184.1 NZ_CP082026:66148-66591 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT214001 NZ_CP109933:1206981-1207241 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References