Detailed information of TA system TA213996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 72383..73026
• Replicon: plasmid pKP18-2073-KPC2
• Accession: NZ_CP082025
• Organism: Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: K6166_RS28305
• Protein ID: WP_001044770.1
• Coordinates: 72383..72799 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K6166_RS28310
• Protein ID: WP_001261282.1
• Coordinates: 72796..73026 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6166_RS28285 (68487)	68487..68759	-	273	Protein_93	transposase	-
K6166_RS28290 (69231)	69231..69232	-	2	WP_024269734.1	IS1 family transposase	-
K6166_RS28295 (69740)	69740..70762	-	1023	WP_000361404.1	helicase UvrD	-
K6166_RS28300 (70747)	70747..72309	-	1563	WP_004206609.1	AAA family ATPase	-
K6166_RS28305 (72383)	72383..72799	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6166_RS28310 (72796)	72796..73026	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6166_RS28315 (72983)	72983..73444	+	462	WP_014343465.1	hypothetical protein	-
K6166_RS28320 (73605)	73605..74549	+	945	WP_011977810.1	hypothetical protein	-
K6166_RS28325 (74586)	74586..74978	+	393	WP_011977811.1	hypothetical protein	-
K6166_RS28330 (75036)	75036..75557	+	522	WP_013214008.1	hypothetical protein	-
K6166_RS28335 (75603)	75603..75806	+	204	WP_011977813.1	hypothetical protein	-
K6166_RS28340 (75836)	75836..76108	+	273	Protein_104	hypothetical protein	-
K6166_RS28345 (76171)	76171..76875	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6166_RS28350 (76941)	76941..77486	+	546	Protein_106	Tn3 family transposase	-
K6166_RS28355 (77522)	77522..77937	-	416	Protein_107	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-187 / blaKPC-2	-	1..130597	130597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T213996 WP_001044770.1 NZ_CP082025:c72799-72383 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T213996 NZ_CP109932:c5736566-5736219 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT213996 WP_001261282.1 NZ_CP082025:c73026-72796 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT213996 NZ_CP109932:c5736827-5736576 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGTCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3