Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5551382..5552007 Replicon chromosome
Accession NZ_CP082024
Organism Klebsiella pneumoniae strain KP18-2073

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6166_RS27470 Protein ID WP_002882817.1
Coordinates 5551382..5551765 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6166_RS27475 Protein ID WP_004150355.1
Coordinates 5551765..5552007 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6166_RS27455 5548748..5549650 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6166_RS27460 5549647..5550282 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6166_RS27465 5550279..5551208 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6166_RS27470 5551382..5551765 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6166_RS27475 5551765..5552007 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6166_RS27480 5552212..5553129 + 918 WP_002882812.1 alpha/beta hydrolase -
K6166_RS27485 5553143..5554084 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6166_RS27490 5554129..5554566 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6166_RS27495 5554563..5555423 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6166_RS27500 5555417..5556016 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213992 WP_002882817.1 NZ_CP082024:c5551765-5551382 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213992 NZ_CP109932:1702656-1702838 [Pseudomonas aeruginosa]
ATGAAGTACAGCGAGTTTCGGCGCTGGTTGAAGGCTCGGGGAGTCATTTTCGAGCCTGCCAAGGGAAGCCACTTCAAGGT
TTACTACGGCGACAACCAGACGATTTTCCCCGACCATGGAGCCAAGGAAATAGGCGATGGGTTGAGGAAGAAGATAATCA
AAGACCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213992 WP_004150355.1 NZ_CP082024:c5552007-5551765 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213992 NZ_CP109932:1702871-1703293 [Pseudomonas aeruginosa]
ATGTTTGACTATCCCGTAACGGTTCACGAGGAAGCCGGCAGCGTGTGGGTCAGCTGCGACGATGTACCTGAAATGGCGAG
CGCCGGAGATACCGTTGATGAAGCGCTGCTGGACGCTGTGGAAGGCCTAGAGTCGGCCCTGTCGCTCTATGTGGACCGGC
GGCAGTCGATCCCGCTGCCGTCGAAAGGCAAAGCGGGTCAGTCGATCGTTCGATTGCCCGCGCTCACTTCGGCCAAGATT
GCGCTGTGGAACACCATGCTGGCTCAGAACGTAGGGAAGGCGGAACTGGCTCGCCGGCTGGGAGTGAATCGCGTTCAGGT
AGACCGCCTGGTGGATCTGCTCCACGGGTCGAAGATCGAAGCGGTGGAACATGCTCTGGCGATCTTGGGCCAACGGTTGG
CTGTGACTGTCATTGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References