Detailed information of TA system TA213971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5307..5887
• Replicon: plasmid pKP18-2050-3
• Accession: NZ_CP082022
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K6159_RS30005
• Protein ID: WP_071177730.1
• Coordinates: 5573..5887 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K6159_RS30000
• Protein ID: WP_000093040.1
• Coordinates: 5307..5585 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS29970	1367..1516	-	150	WP_032440454.1	colicin release lysis protein	-
K6159_RS29975	1601..1858	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
K6159_RS29980	1868..3553	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
K6159_RS29985	3880..4125	+	246	WP_032440458.1	hypothetical protein	-
K6159_RS29990	4399..4770	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
K6159_RS30340	4767..5132	+	366	WP_072354022.1	TonB family protein	-
K6159_RS30000	5307..5585	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K6159_RS30005	5573..5887	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6159_RS30010	6051..6479	+	429	WP_001140599.1	hypothetical protein	-
K6159_RS30015	6505..6684	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K6159_RS30020	6711..7241	-	531	WP_071177729.1	hypothetical protein	-
K6159_RS30025	7248..7979	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
K6159_RS30030	7979..9943	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10049	10049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T213971 WP_071177730.1 NZ_CP082022:5573-5887 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T213971 NZ_CP109929:2604161-2604439 [Escherichia coli O6:K2:H1]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT213971 WP_000093040.1 NZ_CP082022:5307-5585 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT213971 NZ_CP109929:2604439-2604723 [Escherichia coli O6:K2:H1]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1