Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 69825..70735 Replicon plasmid pKP18-2050-1
Accession NZ_CP082021
Organism Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag K6159_RS29120 Protein ID WP_004144067.1
Coordinates 70265..70735 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag K6159_RS29115 Protein ID WP_023287184.1
Coordinates 69825..70268 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6159_RS29095 65241..65990 + 750 Protein_72 IS5 family transposase -
K6159_RS29100 66019..66987 + 969 WP_000654811.1 IS5 family transposase -
K6159_RS29105 67183..68529 + 1347 WP_227532884.1 Mov34/MPN/PAD-1 family protein -
K6159_RS29110 68582..69724 + 1143 Protein_75 translesion error-prone DNA polymerase V subunit UmuC -
K6159_RS29115 69825..70268 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
K6159_RS29120 70265..70735 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
K6159_RS29125 70822..71817 + 996 WP_077254794.1 IS5 family transposase -
K6159_RS29130 71819..72787 + 969 WP_072096747.1 IS5 family transposase -
K6159_RS29135 72976..73236 - 261 WP_017901409.1 hypothetical protein -
K6159_RS29140 73541..74509 + 969 WP_074423483.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA5 / qacE / blaSHV-12 - 1..241489 241489
- inside IScluster/Tn - - 63097..74509 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T213968 WP_004144067.1 NZ_CP082021:70265-70735 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T213968 NZ_CP109929:c1938389-1938015 [Escherichia coli O6:K2:H1]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT213968 WP_023287184.1 NZ_CP082021:69825-70268 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT213968 NZ_CP109929:c1938846-1938478 [Escherichia coli O6:K2:H1]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References