Detailed information of TA system TA213965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 75578..76221
• Replicon: plasmid pKP18-2050-KPC2
• Accession: NZ_CP082020
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: K6159_RS28335
• Protein ID: WP_001044770.1
• Coordinates: 75805..76221 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: K6159_RS28330
• Protein ID: WP_001261282.1
• Coordinates: 75578..75808 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS28295 (70801)	70801..71580	-	780	WP_013214009.1	site-specific integrase	-
K6159_RS28300 (71764)	71764..72768	-	1005	WP_011977814.1	hypothetical protein	-
K6159_RS28305 (72798)	72798..73001	-	204	WP_011977813.1	hypothetical protein	-
K6159_RS28310 (73047)	73047..73568	-	522	WP_013214008.1	hypothetical protein	-
K6159_RS28315 (73626)	73626..74018	-	393	WP_011977811.1	hypothetical protein	-
K6159_RS28320 (74055)	74055..74999	-	945	WP_011977810.1	hypothetical protein	-
K6159_RS28325 (75160)	75160..75621	-	462	WP_014343465.1	hypothetical protein	-
K6159_RS28330 (75578)	75578..75808	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6159_RS28335 (75805)	75805..76221	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6159_RS28340 (76295)	76295..77857	+	1563	WP_004206609.1	AAA family ATPase	-
K6159_RS28345 (77842)	77842..78864	+	1023	WP_000361404.1	helicase UvrD	-
K6159_RS28350 (79120)	79120..79817	+	698	WP_223174630.1	IS1 family transposase	-
K6159_RS28355 (79846)	79846..80118	+	273	Protein_106	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..135837	135837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T213965 WP_001044770.1 NZ_CP082020:75805-76221 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T213965 NZ_CP109929:1218284-1218595 [Escherichia coli O6:K2:H1]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT213965 WP_001261282.1 NZ_CP082020:75578-75808 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT213965 NZ_CP109929:1218592-1219011 [Escherichia coli O6:K2:H1]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3