213961

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5550581..5551206 Replicon chromosome
Accession NZ_CP082019
Organism Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6159_RS27470 Protein ID WP_002882817.1
Coordinates 5550581..5550964 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6159_RS27475 Protein ID WP_004150355.1
Coordinates 5550964..5551206 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6159_RS27455 5547947..5548849 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6159_RS27460 5548846..5549481 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6159_RS27465 5549478..5550407 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6159_RS27470 5550581..5550964 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6159_RS27475 5550964..5551206 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6159_RS27480 5551411..5552328 + 918 WP_002882812.1 alpha/beta hydrolase -
K6159_RS27485 5552342..5553283 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6159_RS27490 5553328..5553765 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6159_RS27495 5553762..5554622 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6159_RS27500 5554616..5555215 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213961 WP_002882817.1 NZ_CP082019:c5550964-5550581 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213961 NZ_CP109929:198308-198415 [Escherichia coli O6:K2:H1]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213961 WP_004150355.1 NZ_CP082019:c5551206-5550964 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213961 NZ_CP109929:c198259-198196 [Escherichia coli O6:K2:H1]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References