Detailed information of TA system TA213959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5143432..5144102
• Replicon: chromosome
• Accession: NZ_CP082019
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6159_RS25500
• Protein ID: WP_004213072.1
• Coordinates: 5143432..5143875 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6159_RS25505
• Protein ID: WP_004213073.1
• Coordinates: 5143872..5144102 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS25465	5138853..5139119	+	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-
K6159_RS25470	5139182..5139673	+	492	WP_011251327.1	DM13 domain-containing protein	-
K6159_RS25475	5139722..5140642	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6159_RS25480	5140733..5141136	+	404	Protein_5005	GAF domain-containing protein	-
K6159_RS25485	5141654..5142288	-	635	Protein_5006	mucoid phenotype regulator RmpA2	-
K6159_RS25490	5142705..5143009	+	305	Protein_5007	transposase	-
K6159_RS30255	5143032..5143283	-	252	WP_186987481.1	hypothetical protein	-
K6159_RS25500	5143432..5143875	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6159_RS25505	5143872..5144102	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6159_RS25510	5144710..5145843	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6159_RS25515	5145859..5146152	+	294	WP_004213076.1	hypothetical protein	-
K6159_RS25520	5146142..5146348	-	207	WP_004213077.1	hypothetical protein	-
K6159_RS25525	5146700..5146990	+	291	WP_004213078.1	hypothetical protein	-
K6159_RS25530	5146980..5147879	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaSHV-187 / aph(3')-Ia / tet(A)	iucA / iucB / iucC / iucD / iutA / rmpA	5049220..5247472	198252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T213959 WP_004213072.1 NZ_CP082019:c5143875-5143432 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T213959 NZ_CP109924:4471620-4471997 [Escherichia coli O125ac:K+:H10]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT213959 WP_004213073.1 NZ_CP082019:c5144102-5143872 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213959 NZ_CP109924:4471204-4471530 [Escherichia coli O125ac:K+:H10]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620