Detailed information of TA system TA213958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5065212..5065738
• Replicon: chromosome
• Accession: NZ_CP082019
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: K6159_RS25095
• Protein ID: WP_000323025.1
• Coordinates: 5065451..5065738 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: K6159_RS25090
• Protein ID: WP_000534858.1
• Coordinates: 5065212..5065451 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS25065	5060274..5060978	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
K6159_RS25070	5061210..5062550	+	1341	WP_000589001.1	ISNCY family transposase	-
K6159_RS25075	5062971..5063282	+	312	Protein_4923	transposase	-
K6159_RS25085	5064626..5064949	+	324	WP_224948387.1	cation transporter dimerization domain-containing protein	-
K6159_RS30240	5065083..5065187	-	105	Protein_4926	hypothetical protein	-
K6159_RS25090	5065212..5065451	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
K6159_RS25095	5065451..5065738	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
K6159_RS25100	5065810..5065968	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
K6159_RS25105	5066571..5067545	+	975	WP_004181899.1	hypothetical protein	-
K6159_RS25110	5067743..5068120	-	378	WP_004181901.1	hypothetical protein	-
K6159_RS25115	5068810..5069877	+	1068	WP_004181903.1	hypothetical protein	-
K6159_RS25120	5070033..5070380	+	348	WP_004181904.1	hypothetical protein	-
K6159_RS25125	5070459..5070692	+	234	WP_004181905.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaSHV-187 / aph(3')-Ia / tet(A)	iucA / iucB / iucC / iucD / iutA / rmpA	5049220..5247472	198252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T213958 WP_000323025.1 NZ_CP082019:5065451-5065738 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T213958 NZ_CP109924:c4313999-4313649 [Escherichia coli O125ac:K+:H10]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCCACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT213958 WP_000534858.1 NZ_CP082019:5065212-5065451 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT213958 NZ_CP109924:c4314244-4313993 [Escherichia coli O125ac:K+:H10]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8