Detailed information of TA system TA213941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1404..1878
• Replicon: plasmid pKP18-2050-4
• Accession: NZ_CP082018
• Organism: Klebsiella pneumoniae strain KP18-2050

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K6159_RS00020
• Protein ID: WP_004197762.1
• Coordinates: 1612..1878 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K6159_RS00015
• Protein ID: WP_000879771.1
• Coordinates: 1404..1628 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6159_RS00005	9..518	+	510	WP_032433972.1	MbeB family mobilization protein	-
K6159_RS00010	525..737	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K6159_RS30345	1113..1241	-	129	WP_000523570.1	hypothetical protein	-
K6159_RS00015	1404..1628	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K6159_RS00020	1612..1878	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6159_RS00025	1948..2298	-	351	WP_004197756.1	hypothetical protein	-
K6159_RS00030	2364..2909	-	546	WP_023317533.1	hypothetical protein	-
K6159_RS00035	2935..3261	-	327	WP_050516389.1	hypothetical protein	-
K6159_RS00040	4417..4593	+	177	WP_004197757.1	hypothetical protein	-
K6159_RS00045	4583..4927	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
K6159_RS30035	5109..5354	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T213941 WP_004197762.1 NZ_CP082018:1612-1878 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T213941 NZ_CP109924:753215-753511 [Escherichia coli O125ac:K+:H10]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT213941 WP_000879771.1 NZ_CP082018:1404-1628 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT213941 NZ_CP109924:753513-753908 [Escherichia coli O125ac:K+:H10]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5