Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94039..94682 Replicon plasmid pKP18-238-KPC2
Accession NZ_CP082015
Organism Klebsiella pneumoniae strain KP18-238

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6162_RS28320 Protein ID WP_001044770.1
Coordinates 94039..94455 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6162_RS28325 Protein ID WP_001261282.1
Coordinates 94452..94682 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6162_RS28300 (90143) 90143..90415 - 273 Protein_124 transposase -
K6162_RS28305 (90887) 90887..90888 - 2 WP_024269734.1 IS1 family transposase -
K6162_RS28310 (91396) 91396..92418 - 1023 WP_000361404.1 helicase UvrD -
K6162_RS28315 (92403) 92403..93965 - 1563 WP_004206609.1 AAA family ATPase -
K6162_RS28320 (94039) 94039..94455 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6162_RS28325 (94452) 94452..94682 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6162_RS28330 (94639) 94639..95100 + 462 WP_014343465.1 hypothetical protein -
K6162_RS28335 (95261) 95261..96205 + 945 WP_011977810.1 hypothetical protein -
K6162_RS28340 (96242) 96242..96634 + 393 WP_011977811.1 hypothetical protein -
K6162_RS28345 (96692) 96692..97213 + 522 WP_013214008.1 hypothetical protein -
K6162_RS28350 (97259) 97259..97462 + 204 WP_011977813.1 hypothetical protein -
K6162_RS28355 (97492) 97492..98496 + 1005 WP_011977814.1 hypothetical protein -
K6162_RS28360 (98680) 98680..99459 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid catA2 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..128480 128480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T213939 WP_001044770.1 NZ_CP082015:c94455-94039 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T213939 NZ_CP109924:c643163-642699 [Escherichia coli O125ac:K+:H10]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGATGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213939 WP_001261282.1 NZ_CP082015:c94682-94452 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213939 NZ_CP109924:c643498-643163 [Escherichia coli O125ac:K+:H10]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References