Detailed information of TA system TA213919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2358699..2359369
• Replicon: chromosome
• Accession: NZ_CP082014
• Organism: Klebsiella pneumoniae strain KP18-238

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6162_RS11420
• Protein ID: WP_004213072.1
• Coordinates: 2358926..2359369 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6162_RS11415
• Protein ID: WP_004213073.1
• Coordinates: 2358699..2358929 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6162_RS11390	2354922..2355821	-	900	WP_004225022.1	nucleotide-binding protein	-
K6162_RS11395	2355811..2356101	-	291	WP_004213078.1	hypothetical protein	-
K6162_RS11400	2356453..2356659	+	207	WP_004213077.1	hypothetical protein	-
K6162_RS11405	2356649..2356942	-	294	WP_004213076.1	hypothetical protein	-
K6162_RS11410	2356958..2358091	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6162_RS11415	2358699..2358929	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6162_RS11420	2358926..2359369	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6162_RS29235	2359518..2359769	+	252	WP_186987481.1	hypothetical protein	-
K6162_RS11430	2359792..2360096	-	305	Protein_2206	transposase	-
K6162_RS11435	2360513..2361147	+	635	Protein_2207	mucoid phenotype regulator RmpA2	-
K6162_RS11440	2361665..2362068	-	404	Protein_2208	GAF domain-containing protein	-
K6162_RS11445	2362159..2363079	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6162_RS11450	2363128..2363619	-	492	WP_011251327.1	DM13 domain-containing protein	-
K6162_RS11455	2363682..2363948	-	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mph(E)	rmpA / iutA / iucD / iucC / iucB / iucA	2311878..2433639	121761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T213919 WP_004213072.1 NZ_CP082014:2358926-2359369 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T213919 NZ_CP109920:c5927347-5927069 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT213919 WP_004213073.1 NZ_CP082014:2358699-2358929 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213919 NZ_CP109920:c5927057-5926752 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620