Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1982412..1983037 Replicon chromosome
Accession NZ_CP082014
Organism Klebsiella pneumoniae strain KP18-238

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6162_RS09635 Protein ID WP_002882817.1
Coordinates 1982654..1983037 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6162_RS09630 Protein ID WP_004150355.1
Coordinates 1982412..1982654 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6162_RS09605 1978403..1979002 + 600 WP_004151865.1 glucose-1-phosphatase -
K6162_RS09610 1978996..1979856 + 861 WP_002882807.1 virulence factor BrkB family protein -
K6162_RS09615 1979853..1980290 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6162_RS09620 1980335..1981276 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6162_RS09625 1981290..1982207 - 918 WP_002882812.1 alpha/beta hydrolase -
K6162_RS09630 1982412..1982654 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6162_RS09635 1982654..1983037 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6162_RS09640 1983211..1984140 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6162_RS09645 1984137..1984772 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6162_RS09650 1984769..1985671 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213917 WP_002882817.1 NZ_CP082014:1982654-1983037 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213917 NZ_CP109920:c4944436-4944146 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213917 WP_004150355.1 NZ_CP082014:1982412-1982654 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213917 NZ_CP109920:c4944732-4944433 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References