Detailed information of TA system TA213903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5135847..5136517
• Replicon: chromosome
• Accession: NZ_CP082010
• Organism: Klebsiella pneumoniae strain KP18-41

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: K6147_RS25425
• Protein ID: WP_004213072.1
• Coordinates: 5135847..5136290 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: K6147_RS25430
• Protein ID: WP_004213073.1
• Coordinates: 5136287..5136517 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6147_RS25390	5131268..5131534	+	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-
K6147_RS25395	5131597..5132088	+	492	WP_011251327.1	DM13 domain-containing protein	-
K6147_RS25400	5132137..5133057	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
K6147_RS25405	5133148..5133551	+	404	Protein_4989	GAF domain-containing protein	-
K6147_RS25410	5134069..5134703	-	635	Protein_4990	mucoid phenotype regulator RmpA2	-
K6147_RS25415	5135120..5135424	+	305	Protein_4991	transposase	-
K6147_RS29060	5135447..5135698	-	252	WP_186987481.1	hypothetical protein	-
K6147_RS25425	5135847..5136290	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
K6147_RS25430	5136287..5136517	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K6147_RS25435	5137125..5138258	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
K6147_RS25440	5138274..5138567	+	294	WP_004213076.1	hypothetical protein	-
K6147_RS25445	5138557..5138763	-	207	WP_004213077.1	hypothetical protein	-
K6147_RS25450	5139115..5139405	+	291	WP_004213078.1	hypothetical protein	-
K6147_RS25455	5139395..5140294	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia / blaSHV-187	iucA / iucB / iucC / iucD / iutA / rmpA	5041785..5237719	195934

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T213903 WP_004213072.1 NZ_CP082010:c5136290-5135847 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T213903 NZ_CP109918:c4914027-4913680 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT213903 WP_004213073.1 NZ_CP082010:c5136517-5136287 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213903 NZ_CP109918:c4914288-4914037 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGATCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTCCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620