Detailed information of TA system TA213885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 211..685
• Replicon: plasmid pKP18-41-3
• Accession: NZ_CP082009
• Organism: Klebsiella pneumoniae strain KP18-41

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: K6147_RS00010
• Protein ID: WP_004197762.1
• Coordinates: 211..477 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: K6147_RS00015
• Protein ID: WP_000879771.1
• Coordinates: 461..685 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6147_RS00010	211..477	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6147_RS00015	461..685	-	225	WP_000879771.1	DUF6290 family protein	Antitoxin
K6147_RS29120	848..976	+	129	WP_000523570.1	hypothetical protein	-
K6147_RS00020	1352..1564	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
K6147_RS00025	1571..2080	-	510	WP_032433972.1	MbeB family mobilization protein	-
K6147_RS29125	2115..2549	-	435	Protein_6	relaxase/mobilization nuclease domain-containing protein	-
K6147_RS00035	2758..3102	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
K6147_RS00040	3092..3268	-	177	WP_004197757.1	hypothetical protein	-
K6147_RS00045	4424..4750	+	327	WP_050516389.1	hypothetical protein	-
K6147_RS00050	4776..5321	+	546	WP_023317533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T213885 WP_004197762.1 NZ_CP082009:c477-211 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T213885 NZ_CP109897:457430-457711 [Xylella fastidiosa subsp. fastidiosa]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT213885 WP_000879771.1 NZ_CP082009:c685-461 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT213885 NZ_CP109897:457722-457997 [Xylella fastidiosa subsp. fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Structures

Antitoxin

• AlphaFold DB: S1EJF5