Detailed information of TA system TA213884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1988..2568
• Replicon: plasmid pKP18-8-4
• Accession: NZ_CP082008
• Organism: Klebsiella pneumoniae strain KP18-8

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K6148_RS28345
• Protein ID: WP_071177730.1
• Coordinates: 2254..2568 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K6148_RS28340
• Protein ID: WP_000093040.1
• Coordinates: 1988..2266 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6148_RS28325	561..806	+	246	WP_032440458.1	hypothetical protein	-
K6148_RS28330	1080..1451	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
K6148_RS28570	1448..1813	+	366	WP_072354022.1	TonB family protein	-
K6148_RS28340	1988..2266	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K6148_RS28345	2254..2568	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K6148_RS28350	2732..3160	+	429	WP_001140599.1	hypothetical protein	-
K6148_RS28355	3186..3365	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K6148_RS28360	3392..3922	-	531	WP_071177729.1	hypothetical protein	-
K6148_RS28365	3929..4660	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
K6148_RS28370	4660..6624	-	1965	WP_222698189.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T213884 WP_071177730.1 NZ_CP082008:2254-2568 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T213884 NZ_CP109894:c2507469-2507170 [Xylella fastidiosa subsp. fastidiosa]
ATGACCTACACGCTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCGGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT213884 WP_000093040.1 NZ_CP082008:1988-2266 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT213884 NZ_CP109894:c2507166-2506858 [Xylella fastidiosa subsp. fastidiosa]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTTGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCCCTTCGGCCTGGTAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1