Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 81138..81874 Replicon plasmid pKP18-8-1
Accession NZ_CP082007
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag K6148_RS27840 Protein ID WP_003026803.1
Coordinates 81392..81874 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K6148_RS27835 Protein ID WP_003026799.1
Coordinates 81138..81404 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS27790 (77200) 77200..77562 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
K6148_RS27795 (77612) 77612..77962 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K6148_RS27800 (78320) 78320..78589 + 270 WP_004152102.1 hypothetical protein -
K6148_RS27805 (78577) 78577..79152 + 576 WP_004152103.1 hypothetical protein -
K6148_RS27810 (79183) 79183..79677 + 495 WP_004152104.1 DNA-binding protein -
K6148_RS27815 (79721) 79721..80089 + 369 WP_004152105.1 hypothetical protein -
K6148_RS27820 (80123) 80123..80326 + 204 WP_004152106.1 HHA domain-containing protein -
K6148_RS27825 (80375) 80375..80632 + 258 WP_004152107.1 hypothetical protein -
K6148_RS27830 (80708) 80708..80962 + 255 WP_004152108.1 hypothetical protein -
K6148_RS27835 (81138) 81138..81404 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K6148_RS27840 (81392) 81392..81874 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
K6148_RS27845 (82075) 82075..83478 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
K6148_RS27850 (83507) 83507..84139 - 633 WP_001567369.1 hypothetical protein -
K6148_RS27855 (84369) 84369..85715 + 1347 WP_020314316.1 ISNCY family transposase -
K6148_RS28625 (85876) 85876..86007 + 132 WP_004218042.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia - 1..174262 174262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T213883 WP_003026803.1 NZ_CP082007:81392-81874 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T213883 NZ_CP109894:c2507166-2506858 [Xylella fastidiosa subsp. fastidiosa]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTTGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCCCTTCGGCCTGGTAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT213883 WP_003026799.1 NZ_CP082007:81138-81404 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT213883 NZ_CP109894:c2507469-2507170 [Xylella fastidiosa subsp. fastidiosa]
ATGACCTACACGCTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCGGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References