Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5352223..5352848 Replicon chromosome
Accession NZ_CP082005
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6148_RS26425 Protein ID WP_002882817.1
Coordinates 5352223..5352606 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6148_RS26430 Protein ID WP_004150355.1
Coordinates 5352606..5352848 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS26410 (5349589) 5349589..5350491 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6148_RS26415 (5350488) 5350488..5351123 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6148_RS26420 (5351120) 5351120..5352049 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6148_RS26425 (5352223) 5352223..5352606 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6148_RS26430 (5352606) 5352606..5352848 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6148_RS26435 (5353053) 5353053..5353970 + 918 WP_004181612.1 alpha/beta hydrolase -
K6148_RS26440 (5353984) 5353984..5354925 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6148_RS26445 (5354970) 5354970..5355407 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6148_RS26450 (5355404) 5355404..5356264 - 861 WP_004146232.1 virulence factor BrkB family protein -
K6148_RS26455 (5356258) 5356258..5356857 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213882 WP_002882817.1 NZ_CP082005:c5352606-5352223 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213882 NZ_CP109894:c1582379-1582125 [Xylella fastidiosa subsp. fastidiosa]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGACTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCTCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213882 WP_004150355.1 NZ_CP082005:c5352848-5352606 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213882 NZ_CP109894:c1582128-1581793 [Xylella fastidiosa subsp. fastidiosa]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGATAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References