Detailed information of TA system TA021388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1216197..1216734
• Replicon: chromosome
• Accession: NC_007650
• Organism: Burkholderia thailandensis E264

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BTH_RS05365
• Protein ID: WP_004549731.1
• Coordinates: 1216197..1216466 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: BTH_RS05370
• Protein ID: WP_011401115.1
• Coordinates: 1216450..1216734 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTH_RS05330	1211574..1212395	+	822	WP_009896491.1	hypothetical protein	-
BTH_RS05335	1212392..1212652	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BTH_RS05340	1212806..1213798	+	993	WP_015967393.1	hypothetical protein	-
BTH_RS05345	1213795..1214346	+	552	WP_011401112.1	DUF1064 domain-containing protein	-
BTH_RS05350	1214343..1214699	+	357	WP_009896497.1	hypothetical protein	-
BTH_RS29935	1215139..1215471	+	333	WP_011401114.1	hypothetical protein	-
BTH_RS05355	1215447..1215707	+	261	WP_009896498.1	hypothetical protein	-
BTH_RS05360	1215704..1216147	+	444	WP_009896499.1	hypothetical protein	-
BTH_RS05365	1216197..1216466	+	270	WP_004549731.1	BrnT family toxin	Toxin
BTH_RS05370	1216450..1216734	+	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
BTH_RS05375	1217365..1217844	+	480	WP_025404174.1	terminase small subunit	-
BTH_RS05380	1217844..1219586	+	1743	WP_011401117.1	terminase large subunit	-
BTH_RS05385	1219594..1220889	+	1296	WP_009896509.1	phage portal protein	-
BTH_RS05390	1220867..1221709	+	843	WP_011401119.1	Clp protease ClpP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1194774..1241408	46634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T21388 WP_004549731.1 NC_007650:1216197-1216466 [Burkholderia thailandensis E264]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T21388 NC_007650:1216197-1216466 [Burkholderia thailandensis E264]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT21388 WP_011401115.1 NC_007650:1216450-1216734 [Burkholderia thailandensis E264]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT21388 NC_007650:1216450-1216734 [Burkholderia thailandensis E264]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTACGACGTCGACATCGTGGACGCCTTCAAGGCAACAGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: Q2T6G1