Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4772854..4773664 Replicon chromosome
Accession NZ_CP082005
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag K6148_RS23745 Protein ID WP_004178461.1
Coordinates 4772854..4773387 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag K6148_RS23750 Protein ID WP_002887278.1
Coordinates 4773398..4773664 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS23740 (4771685) 4771685..4772806 + 1122 WP_002887282.1 cupin domain-containing protein -
K6148_RS23745 (4772854) 4772854..4773387 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
K6148_RS23750 (4773398) 4773398..4773664 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
K6148_RS23755 (4773767) 4773767..4775200 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
K6148_RS23760 (4775190) 4775190..4775873 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
K6148_RS23765 (4776045) 4776045..4777430 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
K6148_RS23770 (4777448) 4777448..4777792 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T213879 WP_004178461.1 NZ_CP082005:c4773387-4772854 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T213879 NZ_CP109894:1389099-1389395 [Xylella fastidiosa subsp. fastidiosa]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT213879 WP_002887278.1 NZ_CP082005:c4773664-4773398 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT213879 NZ_CP109894:1389399-1389704 [Xylella fastidiosa subsp. fastidiosa]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References