Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4137723..4138342 Replicon chromosome
Accession NZ_CP082005
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6148_RS20740 Protein ID WP_002892050.1
Coordinates 4138124..4138342 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6148_RS20735 Protein ID WP_002892066.1
Coordinates 4137723..4138097 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS20725 (4132875) 4132875..4134068 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6148_RS20730 (4134091) 4134091..4137237 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K6148_RS20735 (4137723) 4137723..4138097 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6148_RS20740 (4138124) 4138124..4138342 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6148_RS20745 (4138501) 4138501..4139067 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6148_RS20750 (4139039) 4139039..4139179 - 141 WP_004147370.1 hypothetical protein -
K6148_RS20755 (4139200) 4139200..4139670 + 471 WP_002892026.1 YlaC family protein -
K6148_RS20760 (4139645) 4139645..4141096 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6148_RS20765 (4141197) 4141197..4141895 + 699 WP_002892021.1 GNAT family protein -
K6148_RS20770 (4141892) 4141892..4142032 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6148_RS20775 (4142032) 4142032..4142295 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213878 WP_002892050.1 NZ_CP082005:4138124-4138342 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T213878 NZ_CP109894:1308064-1308345 [Xylella fastidiosa subsp. fastidiosa]
ATGCGGAGGATTAGCCAGACTGGCCAATTCAAGCGCGACTACAAGCGCGAAGCTAAGGGCCAGCATCGCGCCACGCTTGA
TGAGGAACTGATACACGTCTTGGAAGCCTTGACCTGTGATCATCCGTTGGAGCCACGCCACCACGATCACGCACTAACTG
GCGACTGGAAAGATCACCGTGATTGCCACATCAAACCCGACTTGGTACTGATATACCGGAAGCCCGACAACGAGACATTG
CAGCTCGTGCGCATTGGCTCACACAGCGAACTAGGTTTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT213878 WP_002892066.1 NZ_CP082005:4137723-4138097 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT213878 NZ_CP109894:1307814-1308077 [Xylella fastidiosa subsp. fastidiosa]
ATGTCTGCAAACGCAGTTGTCCGTGCCCGCGTTGACGCGCACATCAAAGAAGAAGCATCAACCGTGCTGGCGACGATGGG
CCTAAGCGTGTCTGATGCGTTCCGTATGATGCTGACCCGCATTGCCCGCGAGAAGGCGCTGCCGTTCGAGCCGCTGGTGC
CGAACGAAACTACCATCGCAGCCATGCGTGAAGCCCGTGCTGGTCACCTCAAGAGCTTTGATAGTATTGAGGCGCTCATG
GCGGACTTGCATGCGGAGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References