Detailed information of TA system TA213864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 341195..341781
• Replicon: chromosome
• Accession: NZ_CP082005
• Organism: Klebsiella pneumoniae strain KP18-8

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: K6148_RS02240
• Protein ID: WP_002920800.1
• Coordinates: 341413..341781 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: K6148_RS02235
• Protein ID: WP_004174006.1
• Coordinates: 341195..341416 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6148_RS02215 (337352)	337352..338278	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
K6148_RS02220 (338275)	338275..339552	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
K6148_RS02225 (339549)	339549..340316	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
K6148_RS02230 (340318)	340318..341031	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
K6148_RS02235 (341195)	341195..341416	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K6148_RS02240 (341413)	341413..341781	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
K6148_RS02245 (342054)	342054..343370	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
K6148_RS02250 (343477)	343477..344364	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
K6148_RS02255 (344361)	344361..345206	+	846	WP_004181473.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
K6148_RS02260 (345208)	345208..346278	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	330837..347015	16178

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T213864 WP_002920800.1 NZ_CP082005:341413-341781 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T213864 NZ_CP109891:457731-458033 [Xylella fastidiosa subsp. fastidiosa]
ATGGAGAAAGGCACATCTCACTATAAGCTACCAGTCGTCAAAGCCTTGGTGAAAGCTGGCAAGGTCAGGGCTACAGCGAC
CGCCTACCAAGGAGCACGTGAGCTCGGCATCAAAGATTTAACTGGAATGTGCACCGTTGTCTTGGCGCTCACGTCTGCCG
ACTTCTACAAGAGCATGACGACCTACGCGGATCACACAGTATTCCAGGATGTGTACCACGCCAAGACGGCAAGCGGTGAT
GAGGTGTACCTGAAGTTAACTGTCATTGATGACGTGTTGATTGTTTCCTTCAAGGAGTTATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT213864 WP_004174006.1 NZ_CP082005:341195-341416 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT213864 NZ_CP109891:457368-457643 [Xylella fastidiosa subsp. fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7