Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 6977..7620 Replicon plasmid pKP18-8-KPC2
Accession NZ_CP082004
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K6148_RS00060 Protein ID WP_001044770.1
Coordinates 7204..7620 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K6148_RS00055 Protein ID WP_001261282.1
Coordinates 6977..7207 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS28385 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
K6148_RS00020 (2940) 2940..3734 - 795 WP_004197635.1 site-specific integrase -
K6148_RS00025 (3932) 3932..4948 - 1017 WP_017899884.1 hypothetical protein -
K6148_RS00030 (4959) 4959..5273 - 315 WP_053389906.1 hypothetical protein -
K6148_RS00035 (5300) 5300..5695 - 396 WP_017899885.1 hypothetical protein -
K6148_RS00040 (5864) 5864..6169 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
K6148_RS00045 (6171) 6171..6389 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
K6148_RS00050 (6559) 6559..7020 - 462 WP_160880186.1 hypothetical protein -
K6148_RS00055 (6977) 6977..7207 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K6148_RS00060 (7204) 7204..7620 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K6148_RS00065 (7694) 7694..9256 + 1563 WP_004206609.1 AAA family ATPase -
K6148_RS00070 (9241) 9241..10263 + 1023 WP_000361404.1 helicase UvrD -
K6148_RS00075 (10519) 10519..11216 + 698 WP_223174630.1 IS1 family transposase -
K6148_RS00080 (11245) 11245..11517 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..105913 105913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T213863 WP_001044770.1 NZ_CP082004:7204-7620 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T213863 NZ_CP109891:457076-457357 [Xylella fastidiosa subsp. fastidiosa]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTCAAGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCACTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTATCACTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213863 WP_001261282.1 NZ_CP082004:6977-7207 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213863 NZ_CP109891:457368-457643 [Xylella fastidiosa subsp. fastidiosa]
ATGAAACGTATGCACAATCCCGCACATCCAGGTGAAGTGTTGCGTGAGTATCTTGGTGAGCTCACTGTGACCAATGCGGC
TGCAAAGCTAGGCGTCAGCCGTGTGGCTTTGTCGCGTATTCTCAATGGTACCAATGGCATCTCTGCCGATATGGCCCTTC
GCCTGGAAGATGCGCTTGGCACCAGTGCAGAGATGTGGGCAGCCATGCAGTTCAAGTATGACCTGTGGGTTGCTAGTCAG
CAGCCACGCCCGAAGATCACGCGCCTTCATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References