Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5864..6389 Replicon plasmid pKP18-8-KPC2
Accession NZ_CP082004
Organism Klebsiella pneumoniae strain KP18-8

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag K6148_RS00040 Protein ID WP_013023785.1
Coordinates 5864..6169 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag K6148_RS00045 Protein ID WP_001568025.1
Coordinates 6171..6389 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6148_RS00010 (1551) 1551..2177 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
K6148_RS28385 (2174) 2174..2476 + 303 WP_004197636.1 hypothetical protein -
K6148_RS00020 (2940) 2940..3734 - 795 WP_004197635.1 site-specific integrase -
K6148_RS00025 (3932) 3932..4948 - 1017 WP_017899884.1 hypothetical protein -
K6148_RS00030 (4959) 4959..5273 - 315 WP_053389906.1 hypothetical protein -
K6148_RS00035 (5300) 5300..5695 - 396 WP_017899885.1 hypothetical protein -
K6148_RS00040 (5864) 5864..6169 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
K6148_RS00045 (6171) 6171..6389 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K6148_RS00050 (6559) 6559..7020 - 462 WP_160880186.1 hypothetical protein -
K6148_RS00055 (6977) 6977..7207 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
K6148_RS00060 (7204) 7204..7620 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
K6148_RS00065 (7694) 7694..9256 + 1563 WP_004206609.1 AAA family ATPase -
K6148_RS00070 (9241) 9241..10263 + 1023 WP_000361404.1 helicase UvrD -
K6148_RS00075 (10519) 10519..11216 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..105913 105913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T213862 WP_013023785.1 NZ_CP082004:c6169-5864 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T213862 NZ_CP109890:c2487123-2486824 [Xylella fastidiosa subsp. fastidiosa]
ATGACCTACACGCTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCGGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT213862 WP_001568025.1 NZ_CP082004:c6389-6171 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT213862 NZ_CP109890:c2486820-2486512 [Xylella fastidiosa subsp. fastidiosa]
GTGACCATAACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTTGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCCCTTCGGCCTGGTAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References